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Tóm tắt
Nghiên cứu này được thực hiện với mục tiêu xác định được nấm Pyricularia oryzae gây bệnh đạo ôn lúa tại An Giang và đánh giá được hiệu lực của các chế phẩm nano bạc trên nền silica (Ag/SiO2) đối với các chủng nấm gây bệnh này. Nấm P. oryzae được phân lập từ mẫu bệnh thông qua phương pháp đơn bào tử và định danh dựa trên hình thái và trình tự vùng gen ITS. Năm chủng nấm P. oryzae TS1A, TS2B, TT1C, TT2A và TC1B có đặc trưng sợi nấm màu trắng xám, tạo thành các vòng tròn đồng tâm với sắc tố melanin màu nâu đen, bào tử có hai vách ngăn hình nụ sen thon dài, hơi nhọn hai đầu, đối xứng qua trục theo tâm bào tử. Dựa trên trình tự vùng gen ITS chủng nấm TS1A được xác định là P. oryzae. Chế phẩm nano Ag/SiO2-CMC và Ag/SiO2-CTS ức chế hoàn toàn sự phát triển của sợi nấm P. oryzae TS1A từ nồng độ tương ứng 60 và 75 ppm, và Ag/SiO2-TWE ức chế 78,4% sự phát triển sợi nấm P. oryzae TS1A ở nồng độ 90 ppm. Các chế phẩm nano Ag/SiO2 làm thay đổi cấu trúc điển hình của sợi nấm, gây đứt gãy hoặc vỡ. Nghiên cứu đã chứng minh chế phẩm nano Ag/SiO2 có tiềm năng phát triển thành chế phẩm ứng dụng kiểm soát bệnh đạo ôn trong sản xuất lúa.
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Cách trích dẫn
Võ Thị Ngọc Hà. (2025). Định danh nấm gây bệnh đạo ôn lúa (Pyricularia oryzae) tại An Giang và khả năng kiểm soát nấm của nano Ag/SiO₂ trong điều kiện in vitro. Tạp Chí điện tử Khoa học Và công nghệ nông nghiệp, 9(3), 5119–5129. https://doi.org/10.46826/huaf-jasat.v9n3y2025.1302
Chuyên mục
CÂY TRỒNG - THỰC VẬT
Bản quyền @ 2017-2025 bởi Tạp chí điện tử Khoa học và công nghệ nông nghiệp
Tài liệu tham khảo
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Bui, T.T.T., Tran, Q.V., Vo, T.N.H., Do, H.K., Hoang, D., Duy, L.T., Nguyen, T.T., Dang, V.Q., Tran, C.K. (2024). Surface properties-dependent antifungal efficiency of Ag/SiO2 nanocomposites. Journal of Nanoparticle Research 26, 273, 14 pp. DOI: 10.1007/s11051-024-06190-1.
D’Ávila, L.S., De Filippi, M.C.C., Café-Filho, A.C. (2021). Sensitivity of Pyricularia oryzae Populations to Fungicides Over a 26-Year Time Frame in Brazil. Plant Disease 105, 1771–1780. DOI: 10.1094/PDIS-08-20-1806-RE.
Groth, D.E. (2006). Azoxystrobin Rate and Timing Effects on Rice Head Blast Incidence and Rice Grain and Milling Yields. Plant Disease 90, 1055–1058. DOI: 10.1094/PD-90-1055.
Karunakaran, G., Suriyaprabha, R., Manivasakan, P., Yuvakkumar, R., Rajendran, V., Prabu, P., Kannan, N. (2013). Effect of nanosilica and silicon sources on plant growth promoting rhizobacteria, soil nutrients and maize seed germination. IET Nanobiotechnology 7, 70–77. DOI: 10.1049/iet-nbt.2012.0048.
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Longya, A., Talumphai, S., Jantasuriyarat, C. (2020). Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers. Journal of Fungi 6(1), 38, 13 pp. DOI: 10.3390/jof6010038.
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Montaser, A.S., Jlassi, K., Ramadan, M.A., Sleem, A.A., Attia, M.F. (2021). Alginate, gelatin, and carboxymethyl cellulose coated nonwoven fabrics containing antimicrobial AgNPs for skin wound healing in rats. International Journal of Biological Macromolecules, 173, 203–210. DOI: 10.1016/j.ijbiomac.2021.01.123.
Mumtaz, Shumaila, Ali, S., Mumtaz, Samaira, Mughal, T.A., Tahir, H.M., Shakir, H.A. (2023). Chitosan conjugated silver nanoparticles: the versatile antibacterial agents. Polymer Bulletin, 80, 4719–4736. DOI: 10.1007/s00289-022-04321-z.
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Park, S.-Y., Han, K., O’Neill, D.B., Mul, G. (2017). Stability of Ag@SiO 2 core–shell particles in conditions of photocatalytic overall water-splitting. Journal of Energy Chemistry 26, 309–314. DOI: 10.1016/j.jechem.2016.12.010.
Pham, D.C., Nguyen, T.H., Ngoc, U.T.P., Le, N.T.T., Tran, T.V., Nguyen, D.H. (2018). Preparation, Characterization and Antifungal Properties of Chitosan-Silver Nanoparticles Synergize Fungicide Against Pyricularia oryzae. Journal of Nanoscience and Nanotechnology 18, 5299–5305. DOI: 10.1166/jnn.2018.15400.
Pham, N.B.T., Le, V.K.T., Bui, T.T.T., Phan, N.G.L., Tran, Q.V., Nguyen, M.L., Dang, V.Q., Nguyen, T.T., Vo, T.N.H., Tran, C.K. (2021). Improved Synthesis of Ag/SiO2 Colloidal Nanocomposites and Their Antibacterial Activity Against Ralstonia solanacearum 15. Journal of Nanoscience and Nanotechnology, 21, 1598–1605. DOI: 10.1166/jnn.2021.19021.
Prema, P., Thangapandiyan, S., Immanuel, G. (2017). CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. Carbohydrate Polymers, 158, 141–148. DOI: 10.1016/j.carbpol.2016.11.083.
Rangelova, N., Aleksandrov, L., Angelova, T., Georgieva, N., Müller, R. (2014). Preparation and characterization of SiO2/CMC/Ag hybrids with antibacterial properties. Carbohydrate Polymers, 101, 1166–1175. DOI: 10.1016/j.carbpol.2013.10.041.
Salem, S.S., Hashem, A.H., Sallam, A.-A.M., Doghish, A.S., Al-Askar, A.A., Arishi, A.A., Shehabeldine, A.M. (2022). Synthesis of Silver Nanocomposite Based on Carboxymethyl Cellulose: Antibacterial, Antifungal and Anticancer Activities. Polymers, 14 (16), 3352, 16 pp. DOI: 10.3390/polym14163352.
Shi, H., Wen, H., Xie, S., Li, Y., Chen, Y., Liu, Z., Jiang, N., Qiu, J., Zhu, X., Lin, F., Kou, Y., 2023. Antifungal activity and mechanisms of AgNPs and their combination with azoxystrobin against Magnaporthe oryzae. Environmental Science: Nano, 10, 2412–2426. DOI: 10.1039/D3EN00168G.
Tamura, K., Stecher, G., Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, (38), 3022–3027. DOI: 10.1093/molbev/msab120.
Wang, Y., Deng, C., Rawat, S., Cota-Ruiz, K., Medina-Velo, I., Gardea-Torresdey, J.L. (2021). Evaluation of the Effects of Nanomaterials on Rice (Oryza sativa L.) Responses: Underlining the Benefits of Nanotechnology for Agricultural Applications. ACS Agricultural Science & Technology, 1, 44–54. DOI: 10.1021/acsagscitech.1c00030.
Wang, Y., Dimkpa, C., Deng, C., Elmer, W.H., Gardea-Torresdey, J., White, J.C. (2022). Impact of engineered nanomaterials on rice (Oryza sativa L.): A critical review of current knowledge. Environmental Pollution, 297, 118738. DOI : 10.1016/j.envpol.2021.118738.
Xiu, Z., Zhang, Q., Puppala, H.L., Colvin, V.L., Alvarez, P.J.J. (2012). Negligible particle-specific antibacterial activity of silver nanoparticles. Nano Letters, 12, 4271–4275. DOI: 10.1021/nl301934w.
Phi, A. (2023). Current Status of Rice Blast in Vietnam and Future Perspectives. Open Access Library Journal, 10636. DOI: 10.4236/oalib.1110636.
Balouiri, M., Sadiki, M., Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6, 71–79. DOI: 10.1016/j.jpha.2015.11.005.
Bui, T.T.T., Tran, Q.V., Vo, T.N.H., Do, H.K., Hoang, D., Duy, L.T., Nguyen, T.T., Dang, V.Q., Tran, C.K. (2024). Surface properties-dependent antifungal efficiency of Ag/SiO2 nanocomposites. Journal of Nanoparticle Research 26, 273, 14 pp. DOI: 10.1007/s11051-024-06190-1.
D’Ávila, L.S., De Filippi, M.C.C., Café-Filho, A.C. (2021). Sensitivity of Pyricularia oryzae Populations to Fungicides Over a 26-Year Time Frame in Brazil. Plant Disease 105, 1771–1780. DOI: 10.1094/PDIS-08-20-1806-RE.
Groth, D.E. (2006). Azoxystrobin Rate and Timing Effects on Rice Head Blast Incidence and Rice Grain and Milling Yields. Plant Disease 90, 1055–1058. DOI: 10.1094/PD-90-1055.
Karunakaran, G., Suriyaprabha, R., Manivasakan, P., Yuvakkumar, R., Rajendran, V., Prabu, P., Kannan, N. (2013). Effect of nanosilica and silicon sources on plant growth promoting rhizobacteria, soil nutrients and maize seed germination. IET Nanobiotechnology 7, 70–77. DOI: 10.1049/iet-nbt.2012.0048.
Khan, F., Pandey, P., Upadhyay, T.K. (2022). Applications of Nanotechnology-Based Agrochemicals in Food Security and Sustainable Agriculture: An Overview. Agriculture 12, 1672. DOI : 10.3390/agriculture12101672.
Longya, A., Talumphai, S., Jantasuriyarat, C. (2020). Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers. Journal of Fungi 6(1), 38, 13 pp. DOI: 10.3390/jof6010038.
Mew, T.-W., Gonzales, P. (2002). A handbook of rice seedborne fungi. International Rice Research Institute, 83 pp.
Mollahosseini, A., Rahimpour, A., Jahamshahi, M., Peyravi, M., Maryam, K. (2012). The effect of silver nanoparticle size on performance and antibacteriality of polysulfone ultrafiltration membrane. Desalination, 306, 41–50. DOI: 10.1016/j.desal.2012.08.035.
Montaser, A.S., Jlassi, K., Ramadan, M.A., Sleem, A.A., Attia, M.F. (2021). Alginate, gelatin, and carboxymethyl cellulose coated nonwoven fabrics containing antimicrobial AgNPs for skin wound healing in rats. International Journal of Biological Macromolecules, 173, 203–210. DOI: 10.1016/j.ijbiomac.2021.01.123.
Mumtaz, Shumaila, Ali, S., Mumtaz, Samaira, Mughal, T.A., Tahir, H.M., Shakir, H.A. (2023). Chitosan conjugated silver nanoparticles: the versatile antibacterial agents. Polymer Bulletin, 80, 4719–4736. DOI: 10.1007/s00289-022-04321-z.
Nalley, L., Tsiboe, F., Durand-Morat, A., Shew, A., Thoma, G. (2016). Economic and Environmental Impact of Rice Blast Pathogen (Magnaporthe oryzae) Alleviation in the United States. Plos One 11, e0167295. DOI: 10.1371/journal.pone.0167295.
Okey-Onyesolu, C.F., Hassanisaadi, M., Bilal, M., Barani, M., Rahdar, A., Iqbal, J., Kyzas, G.Z. (2021). Nanomaterials as Nanofertilizers and Nanopesticides: An Overview. Chemistry Select 6, 8645–8663. DOI: 10.1002/slct.202102379.
Park, S.-Y., Han, K., O’Neill, D.B., Mul, G. (2017). Stability of Ag@SiO 2 core–shell particles in conditions of photocatalytic overall water-splitting. Journal of Energy Chemistry 26, 309–314. DOI: 10.1016/j.jechem.2016.12.010.
Pham, D.C., Nguyen, T.H., Ngoc, U.T.P., Le, N.T.T., Tran, T.V., Nguyen, D.H. (2018). Preparation, Characterization and Antifungal Properties of Chitosan-Silver Nanoparticles Synergize Fungicide Against Pyricularia oryzae. Journal of Nanoscience and Nanotechnology 18, 5299–5305. DOI: 10.1166/jnn.2018.15400.
Pham, N.B.T., Le, V.K.T., Bui, T.T.T., Phan, N.G.L., Tran, Q.V., Nguyen, M.L., Dang, V.Q., Nguyen, T.T., Vo, T.N.H., Tran, C.K. (2021). Improved Synthesis of Ag/SiO2 Colloidal Nanocomposites and Their Antibacterial Activity Against Ralstonia solanacearum 15. Journal of Nanoscience and Nanotechnology, 21, 1598–1605. DOI: 10.1166/jnn.2021.19021.
Prema, P., Thangapandiyan, S., Immanuel, G. (2017). CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. Carbohydrate Polymers, 158, 141–148. DOI: 10.1016/j.carbpol.2016.11.083.
Rangelova, N., Aleksandrov, L., Angelova, T., Georgieva, N., Müller, R. (2014). Preparation and characterization of SiO2/CMC/Ag hybrids with antibacterial properties. Carbohydrate Polymers, 101, 1166–1175. DOI: 10.1016/j.carbpol.2013.10.041.
Salem, S.S., Hashem, A.H., Sallam, A.-A.M., Doghish, A.S., Al-Askar, A.A., Arishi, A.A., Shehabeldine, A.M. (2022). Synthesis of Silver Nanocomposite Based on Carboxymethyl Cellulose: Antibacterial, Antifungal and Anticancer Activities. Polymers, 14 (16), 3352, 16 pp. DOI: 10.3390/polym14163352.
Shi, H., Wen, H., Xie, S., Li, Y., Chen, Y., Liu, Z., Jiang, N., Qiu, J., Zhu, X., Lin, F., Kou, Y., 2023. Antifungal activity and mechanisms of AgNPs and their combination with azoxystrobin against Magnaporthe oryzae. Environmental Science: Nano, 10, 2412–2426. DOI: 10.1039/D3EN00168G.
Tamura, K., Stecher, G., Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, (38), 3022–3027. DOI: 10.1093/molbev/msab120.
Wang, Y., Deng, C., Rawat, S., Cota-Ruiz, K., Medina-Velo, I., Gardea-Torresdey, J.L. (2021). Evaluation of the Effects of Nanomaterials on Rice (Oryza sativa L.) Responses: Underlining the Benefits of Nanotechnology for Agricultural Applications. ACS Agricultural Science & Technology, 1, 44–54. DOI: 10.1021/acsagscitech.1c00030.
Wang, Y., Dimkpa, C., Deng, C., Elmer, W.H., Gardea-Torresdey, J., White, J.C. (2022). Impact of engineered nanomaterials on rice (Oryza sativa L.): A critical review of current knowledge. Environmental Pollution, 297, 118738. DOI : 10.1016/j.envpol.2021.118738.
Xiu, Z., Zhang, Q., Puppala, H.L., Colvin, V.L., Alvarez, P.J.J. (2012). Negligible particle-specific antibacterial activity of silver nanoparticles. Nano Letters, 12, 4271–4275. DOI: 10.1021/nl301934w.
Phi, A. (2023). Current Status of Rice Blast in Vietnam and Future Perspectives. Open Access Library Journal, 10636. DOI: 10.4236/oalib.1110636.