KHẢ NĂNG KHÁNG OXY HÓA, ỨC CHẾ ENZYME α-AMYLASE VÀ α-GLUCOSIDASE CỦA CAO CHIẾT TỪ LÁ CÂY LỘC VỪNG (Barringtonia acutangula)

Abstract

In this research, the antioxidant role, inhibiting α-amylase and α-glucosidase of Barringtonia acutangula leaves were investigated in vitro. The leave extraction was carried out by soxhlet method with aqueous, ethanol 70% and methanol 70% solvents. The content of phenolic, flavonoid, inhibition activities of α-amylase, α-glucosidase and antioxidant were measured by spectrophotometer at 510 nm, 765 nm, 660 nm, 405 nm, 517 nm wavelength. The results showed that the moisture content was 70.64% and extraction efficiency of B. acutangula leave ranged from 9.78 to 13.13%. The leaves of B. acutangula contains some bioactive compounds such as alkaloids, flavonoids, saponins, terpenoids, steroids, tannin and phenol. The total polyphenol of B. acutangula extract is 70,06 (aqueous); 77,94 (ethanol); 85,23 (methanol) mg GE/g extract. The total flavonoid of B. acutangula extract is 88,91 (aqueous); 109,65 (ethanol); 125,56 (methanol) mg quercetin/g extract. The B. acutangula leaves extract has antioxidant ability by DPPH method with an IC₅₀ values of 121.16 µg/mL (aqueous); 109.60 µg/mL (ethanol) and 98.42 µg/mL (methanol), respectively. The B. acutangula leaves extract has the ability to inhibit α-amylase with an IC₅₀ values of 145.31 µg/mL (aqueous); 131.72 µg/mL (ethanol) and 120.62 µg/mL (methanol), respectively. The B. acutangula leaves extract has the ability to inhibit α-glucosidase with an IC₅₀ values of 197.6 µg/mL (aqueous); 176.73 µg/mL (ethanol) and 158.01 µg/mL (methanol), respectively.