NGHIÊN CỨU PHƯƠNG PHÁP NHÂN GIỐNG IN VITRO CÂY SẮN (Manihot esculenta) ĐỂ SẢN XUẤT CÂY GIỐNG SẠCH BỆNH

Abstract

Conventional cassava propagation using stem cuttings has limitations, including low multiplication rate and the potential for disease transmission from mother plant to offspring. The objective of this study is to produce the disease – free cassava plants using the in vitro propagation. The results indicated that 5% sodium hypochlorite (v/v) at 10 minutes and 20 minutes were effective sterilization for the first to 5^th and 6^th to 10^th nodal explant, respectively. For multiplication, in vitro nodes were cultivated in MS medium with 0 – 5 mg/L BAP. In medium with 1 – 3 mg/L BAP, shoots developed directly from nodes, while in medium with 4 - 5 mg/L BAP, shoots developed from callus after six-week culture, however, regenerated shoots had some abnormalities in morphology. Therefore, for multiplication of elite cassava plants, the single node culture in the MS media with 1mg/L BAP was selected. The cassava in vitro plants developed in MS with 1mg/L BAP and 0,1 mg/L NAA.