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Abstract
Among the serious plant diseases affecting chili plants, anthracnose disease, caused by Colletotrichum spp., is one of the most economically important diseases. In this study, 32 Colletotrichum spp. isolates were revived and subsequently evaluated for virulence on F1 Santa chili plants to confirm the pathogenicity of the fungi after the storage period. Morphological and genetic diversity were assessed based on colony characteristics, spore and appressoria morphology, and ISSR molecular markers. The results showed that all 32 Colletotrichum spp. isolates retained their virulence after storage, causing disease incidence on F1 Santa chili, ranging from 11.1% to 94.4%, and disease severity indices from 3.7% to 47.5%. The isolates were classified into three morphological groups based on colony traits, sporeshape, and appressoria morphology. Using eight ISSR primers, the isolates were further divided into five genetic groups, with genetic similarity coefficients ranging from 0.49 to 0.87, indicating a relatively high level of genetic diversity. This diversity showed correlations with both the morphological characteristics and the geographical origin of the isolates.
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