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Abstract
This study aimed to apply a new bioproduct containing beneficial microorganisms as biocontrol to the yellow leaf and root rot disease of coffee plants in Vietnam caused by pathogenic nematodes (Meloidogyne spp., Pratylenchus spp) and fungi (Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Rhizoctonia bataticola) and focused on evaluating the synergistic effect of nematophagous fungi and antagonistic microorganisms to control the pathogens. The results showed that the population of parasitic nematodes in the coffee-growing soil was reduced by 69.82% when treated with both nematophagous fungi and antagonistic microorganisms, compared to 61% and 67.24% when treated with nematophagous fungi or with antagonistic microorganisms alone. The reduction of the F. oxysporum population was 76.44% when both nematophagous fungi and antagonistic microorganisms were used, compared to 52.17% and 75.53% when treated with nematophagous fungi or with antagonistic microorganisms after 6 months. By using the new bioproduct, the reduction of coffee plant-parasitic nematodes, pathogen fungi, and the rate of the diseased coffee plant was 77.16%, 78.04%, and 79.08% for the coffee plant at the young stage, and 78.12%, 79.26%, and 78.48% for the coffee plant at the maturity stage after 270 days of application. Field demonstrations on the application of new bioproducts showed a disease reduction of 82.3% compared to farmer practices and brought a benefit of 9,400,000 VND/ha, equivalent to an income increase of 21.7% for the farmer. This new bioproduct can be applied to control the yellow leaf and root rot disease complex of coffee plants caused by Pratylenchus spp. and F. oxysporum in Taynguyen.
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How to Cite
Đào Hữu Hiền, Pham Van Toan, Nguyen Thị Hồng Minh, & Ho Hanh. (2026). Synergistic effect of nematophagous fungi and antagonistic microorganisms to control the pathogen caused yellow leaf and root rot disease of coffee in Taynguyen. E-Journal of Agricultural Science and Technology, 10(3), 5723–5736. https://doi.org/10.46826/huaf-jasat.v10n3y2026.1289
Section
PLANTS - CROP SCIENCES
Copyright @ 2017-2026 by E-Journal of Agricultural Science and Technology
References
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Aminuzzaman, F.M., Jahan, S.N., Shammi, J., Mitu, A.I., & Liu, X.Z. (2018). Isolation and screening of fungi associated with eggs and females of root-knot nematodes and their biocontrol potential against Meloidogyne incognita in Bangladesh. Archives of Phytopathology and Plant Protection, 51(5-6), 288–308. DOI: https://doi.org/ 10.1080/03235408.2018.1472359
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Bubici, G. (2018). Streptomyces spp. as biocontrol agents against Fusarium species. CAB Reviews, 13, 50.
Burgess, L.W., Knight, TE., Tesoriero, L., & Phan Thuy Hien. (2009). Diagnostic manual for plant diseases in Vietnam. Australian Center for International Agricultural Research, ACIAR
Dwibedi, V., & Saxena, S. (2019). Diversity and phylogeny of resveratrol-producing culturable endophytic fungi from Vitis species in India. Biotech, 9(5), 182.
Expósito, R.G., de Bruijn, I., Postma, J., & Raaijmakers, J.M. (2017). Current insights into the role of rhizosphere bacteria in disease suppressive soils. Front Micro, 8, 2529.
Ha, T. T. T., & Ton, N. T. (2011). Study on composition and nematode damage on pepper plants at Cam Lo, Quang Tri. Journal of Science, Hue University, 67, 5-12.
Ha, H., Tran, L. H., Nguyen, T. H., Nguyen, D. A. T., Nguyen, H. H. T., Pham, N. B., Trinh, P. Q., de Boer, T., Brouwer, A., & Chu, H. H. (2020). Occurrence of endophytic bacteria in Vietnamese Robusta coffee roots and their effects on plant parasitic nematodes. Symbiosis, 80, 75-84.
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Pal, K.K., & Gardener, B.M. (2006). Biological control of plant pathogens. The Plant Health Instructor, 1-25.
QCVN 01-38:2010/BNNPTNT National technical regulation on surveillance method of plant pests, Hanoi, Vietnam
Singh, U.B., Sahu, A., Sahu, N., Singh, R.K., Renu, S., Singh, D.P., Manna, M.C., Sarma, B.K., Singh, H.B., Singh, K.P. (2012). Arthrobotrys oligospora mediated biological control of diseases of tomato (Lycopersicon esculentum Mill.) caused by Meloidogyne incognita and Rhizoctonia solani. Journal of Applied Microbiology, 114, 196-208
Stirling, G.R., Wong, E., & Bhuiyan, S. (2017). Pasteuria, a bacterial parasite of plant-parasitic nematodes: its occurrence in Australian sugarcane soils and its role as a biological control agent in naturally-infested soil. Australasian Plant Pathology, 46, 563-569
Ta Hong Linh, Nguyen Van Tuat, Nguyen Van Viet, Truong Hong, Nguyen Xuan Hoa (2018). Effect of fungi and nematodes on yellowing leaf and root rot diseases on Robusta coffee under different rotation systems in Central Highland of Vietnam. Journal of Vietnam Agricultural Science and Technology, 3(88), 35-41
Trinh, Q.P., Le, T.M.L., Nguyen, T.D., Nguyen, H.T., Liebanas, G., & Nguyen, T.A.D. (2019). Meloidogyne daklakensis n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with Robusta coffee (Coffea canephora Pierre ex A. Froehner) in the Western Highlands, Vietnam. Journal of Helminthology, 93, 242–254.
Tsay, T.T., Chen, P., Wu, W.S. (2006). A new method for isolating and selecting agents with high antagonistic ability against plant parasitic nematodes. Plant Pathology Bulletin, 15, 9-16
Villain, L., Lima Salgada, S.M., & Phap Quang Trinh. (2018). Nematode parasite in coffee and cocoa. In Sikora RA, Coyne D, Hallmann J, Timper P ed: Plant parasitic nematodes in subtropical and tropical agriculture, CABI, Wallingford, pp. 536–583
Vovlas, N., Di Vito, M. (1991). Effect of root-knot nematodes Meloidogyne incognita and M. javanica on the growth of coffee (Coffea arabica L.) in pots. Nematologia Mediterranea, 19(2), 253-258
Vu, A.T., Trinh, Q.P., Nguyen, V.T. (2014). Nematode in basalt soil replaces coffee and the relationship of nematode with yellow leaf coffee symptoms in Gia Lai. Journal of Agriculture and Agricultural Development, 23, 36–43
Wanjohi, W.J., Wafula, G.O., &Macharia, C.M. (2018). Integrated management of fusarium wilt-root knot nematode complex on tomato in central highlands of Kenya. Sustainable Agriculture Research, 7(2), 8-18.
Zhang, L.N., Wang, D.C., Hu, Q., Dai, X.Q., Xie, Y.S., Li, Q., Liu, H.M. and Guo, J.H. (2019). Consortium of Plant Growth-Promoting Rhizobacteria Strains Suppresses Sweet Pepper Disease by Altering the Rhizosphere Microbiota. Fronties in Microbiology, 10, 1668. DOI: https://doi.org/ 10.3389/fmicb.2019.01668
Ahmad, R.Z., Sidi, B.B., Endrawati, D., Ekawasti, F., & Chaerani (2019). Paecilomyces lilacinus and P. variotii as a predator of nematode and trematode eggs. IOP Conf. Series: Earth and Environmental Science, 299, IOP Publishing. DOI: https://doi.org/ 10.1088/1755-1315/299/1/012056
Aminuzzaman, F.M., Jahan, S.N., Shammi, J., Mitu, A.I., & Liu, X.Z. (2018). Isolation and screening of fungi associated with eggs and females of root-knot nematodes and their biocontrol potential against Meloidogyne incognita in Bangladesh. Archives of Phytopathology and Plant Protection, 51(5-6), 288–308. DOI: https://doi.org/ 10.1080/03235408.2018.1472359
Azam, T., Akhtar, M.S., & Hisamuddin, A. (2013). Histological interactions of Paecilomyces lilacinus with root-knot nematode Meloidogyne incognita and their effect on the growth of tomato. Advanced Science Engineering, 5, 335–341
Bell, C.A., Atkinson, H.J., Andrade, A.C., Nguyen, H.X., Swibawa, I.G., Lilley, C., McCarthy, J., Urwin, P. (2018). A high throughput molecular pipeline reveals the diversity in prevalence and abundance of Pratylenchus and Meloidogyne species in coffee plantations. Phytopathology, 108(5), 641 – 650.
Berendsen, R.L., Vismans, G., Yu, K., Song, Y., de Jonge, R., Burgman, W.P., Burmølle, M., Herschend, J., Bakker, P.A.H.M., & Pieterse, C.M.J. (2018). Disease-induced assemblage of a plant-beneficial bacterial consortium. The ISME Journal, 12, 1496–1507.
Boughalleb, M.H., Salem, N.I.B., & Hamdi, M.M. (2018). Evaluation of the efficiency of Trichoderma, Penicillium, and Aspergillus species as biological control agents against four soil-borne fungi of melon and watermelon. Egyptian Journal of Biological Pest Control, 28(1), 13- 25.
Bubici, G. (2018). Streptomyces spp. as biocontrol agents against Fusarium species. CAB Reviews, 13, 50.
Burgess, L.W., Knight, TE., Tesoriero, L., & Phan Thuy Hien. (2009). Diagnostic manual for plant diseases in Vietnam. Australian Center for International Agricultural Research, ACIAR
Dwibedi, V., & Saxena, S. (2019). Diversity and phylogeny of resveratrol-producing culturable endophytic fungi from Vitis species in India. Biotech, 9(5), 182.
Expósito, R.G., de Bruijn, I., Postma, J., & Raaijmakers, J.M. (2017). Current insights into the role of rhizosphere bacteria in disease suppressive soils. Front Micro, 8, 2529.
Ha, T. T. T., & Ton, N. T. (2011). Study on composition and nematode damage on pepper plants at Cam Lo, Quang Tri. Journal of Science, Hue University, 67, 5-12.
Ha, H., Tran, L. H., Nguyen, T. H., Nguyen, D. A. T., Nguyen, H. H. T., Pham, N. B., Trinh, P. Q., de Boer, T., Brouwer, A., & Chu, H. H. (2020). Occurrence of endophytic bacteria in Vietnamese Robusta coffee roots and their effects on plant parasitic nematodes. Symbiosis, 80, 75-84.
Henderson, C.F., &Tilton, E.W. (1955). Test with acaricides against the brow wheat mite. J. Econ.Entomol, 48, 157-161.
International Coffee Organization. (2020). Coffee Development Report (2020).
Kawabata, A.M., Myers, R., Cho, A., & Nakamoto, S.T. (2018). Kona Coffee Root-Knot Nematode Sampling Procedures. College of Tropical Agriculture and Human Resources
Kung, S.P., Gaugler, R., & Kaya, H.K. (1991). Effects of soil temperature, moisture, and relative humidity on entomopathogenic nematode persistence. Journal of Invertebrate Pathology, 57(2), 242-249.
Le, D.K., Stirling, G.R., Guest, D., & Safianowicz, K. (2018). Coffee parasitic nematodes in Australia and the potential of organic amendments in the management of Pratylenchus coffeae. 10th ASDS, Adelaide, SA, 77-79
Mardanova, A.M., Hadieva, G.F., Lutfullin, M.T., Khilyas, I.V., Minnullina, L.F., Gilyazeva, A.G., Bogomolnaya, LM. and Sharipova, M.R. (2017) Bacillus subtilis strains with antifungal activity against the phytopathogenic Fungi. Agricultural Sciences, 8, 1-20.
Ministry of Agriculture and Rural Development. (2024). Annual report, Hanoi, Vietnam.
Nguyen, T. H. M., Dao, T. T. H., Nguyen, D. T., Nguyen, T. Q., Dao, H. H., Ho, H., Tran, N. K., Nguyen, T. H., Vu, T. N., & Pham, V. T. (2020). Selection to determine the consortium of microorganisms antagonistic to coffee pathogen fungi and parasitic nematode. Science and Technology Journal of Agriculture and Rural Development, 11, 3-10.
Nguyen, V. H., Nguyen, T. H., Tran, T. T. T., & Pham, V. T. (2019). Isolation and selection of Arthrobotrys nematophagous fungi to control the nematodes on coffee and black pepper plants in Vietnam. Archives of Phytopathology and Plant Protection, 52(7–8), 825-843.
Pal, K.K., & Gardener, B.M. (2006). Biological control of plant pathogens. The Plant Health Instructor, 1-25.
QCVN 01-38:2010/BNNPTNT National technical regulation on surveillance method of plant pests, Hanoi, Vietnam
Singh, U.B., Sahu, A., Sahu, N., Singh, R.K., Renu, S., Singh, D.P., Manna, M.C., Sarma, B.K., Singh, H.B., Singh, K.P. (2012). Arthrobotrys oligospora mediated biological control of diseases of tomato (Lycopersicon esculentum Mill.) caused by Meloidogyne incognita and Rhizoctonia solani. Journal of Applied Microbiology, 114, 196-208
Stirling, G.R., Wong, E., & Bhuiyan, S. (2017). Pasteuria, a bacterial parasite of plant-parasitic nematodes: its occurrence in Australian sugarcane soils and its role as a biological control agent in naturally-infested soil. Australasian Plant Pathology, 46, 563-569
Ta Hong Linh, Nguyen Van Tuat, Nguyen Van Viet, Truong Hong, Nguyen Xuan Hoa (2018). Effect of fungi and nematodes on yellowing leaf and root rot diseases on Robusta coffee under different rotation systems in Central Highland of Vietnam. Journal of Vietnam Agricultural Science and Technology, 3(88), 35-41
Trinh, Q.P., Le, T.M.L., Nguyen, T.D., Nguyen, H.T., Liebanas, G., & Nguyen, T.A.D. (2019). Meloidogyne daklakensis n. sp. (Nematoda: Meloidogynidae), a new root-knot nematode associated with Robusta coffee (Coffea canephora Pierre ex A. Froehner) in the Western Highlands, Vietnam. Journal of Helminthology, 93, 242–254.
Tsay, T.T., Chen, P., Wu, W.S. (2006). A new method for isolating and selecting agents with high antagonistic ability against plant parasitic nematodes. Plant Pathology Bulletin, 15, 9-16
Villain, L., Lima Salgada, S.M., & Phap Quang Trinh. (2018). Nematode parasite in coffee and cocoa. In Sikora RA, Coyne D, Hallmann J, Timper P ed: Plant parasitic nematodes in subtropical and tropical agriculture, CABI, Wallingford, pp. 536–583
Vovlas, N., Di Vito, M. (1991). Effect of root-knot nematodes Meloidogyne incognita and M. javanica on the growth of coffee (Coffea arabica L.) in pots. Nematologia Mediterranea, 19(2), 253-258
Vu, A.T., Trinh, Q.P., Nguyen, V.T. (2014). Nematode in basalt soil replaces coffee and the relationship of nematode with yellow leaf coffee symptoms in Gia Lai. Journal of Agriculture and Agricultural Development, 23, 36–43
Wanjohi, W.J., Wafula, G.O., &Macharia, C.M. (2018). Integrated management of fusarium wilt-root knot nematode complex on tomato in central highlands of Kenya. Sustainable Agriculture Research, 7(2), 8-18.
Zhang, L.N., Wang, D.C., Hu, Q., Dai, X.Q., Xie, Y.S., Li, Q., Liu, H.M. and Guo, J.H. (2019). Consortium of Plant Growth-Promoting Rhizobacteria Strains Suppresses Sweet Pepper Disease by Altering the Rhizosphere Microbiota. Fronties in Microbiology, 10, 1668. DOI: https://doi.org/ 10.3389/fmicb.2019.01668