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Abstract
This study explored the chemical constituents and bioactivities of cicada exuviae extracts from Cryptotympana mandarina. Gas chromatography–mass spectrometry (GC-MS) analysis identified 23 diverse compounds, including fatty acids and their esters, aromatic compounds, alkanes, and other constituents. Among these, aromatic compounds and fatty acids with their esters were predominant. In vitro assays revealed that the cicada exuviae extracts exhibited notable antioxidant activity by DPPH and ABTS radical scavenging with IC50 of 0.069-0.752 mg/mL. These extracts also displayed antidiabetic potential, inhibiting α-amylase and α-glucosidase with IC50 of 0.65-3.69 mg/mL. In addition, the cicada exuviae extracts displayed antimicrobial effects against the examined microorganisms with minimum inhibitory concentrations (MICs) ranging from 64 to 512 µg/mL. These findings highlight cicada exuviae extracts as a promising natural source of bioactive compounds with antioxidant, antidiabetic, and antimicrobial properties.
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How to Cite
Vu Thi Thanh Tam, Pham Hong Thai, Le Canh Viet Cuong, & Ton That Huu Dat. (2026). Chemical constituents and biological activities of cicada exuviae extracts from Cryptotympana mandarina. E-Journal of Agricultural Science and Technology, 10(2), 5520–5528. https://doi.org/10.46826/huaf-jasat.v10n2y2026.1319
Section
PLANTS - CROP SCIENCES
Copyright @ 2017-2026 by E-Journal of Agricultural Science and Technology
References
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Xu, M. Z., Lee, W. S., Han, J. M., Oh, H. W., Park, D. S., Tian, G. R., Jeong, T. S., & Park, H. Y. (2006). Antioxidant and anti-inflammatory activities of N-acetyldopamine dimers from Periostracum cicadae. Bioorganic and Medicinal Chemistry, 14(23), 7826-7834. DOI: https://doi.org/10.1016/j.bmc.2006.07.063.
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Casillas-Vargas, G., Ocasio-Malavé, C., Medina, S., Morales-Guzmán, C., Del Valle, R. G., Carballeira, N. M., & Sanabria-Ríos, D. J. (2021). Antibacterial fatty acids: An update of possible mechanisms of action and implications in the development of the next-generation of antibacterial agents. Progress in Lipid Research, 82, 101093. DOI: https://doi.org/10.1016/j.plipres.2021.101093.
Chang, T. M., Tsen, J. H., Yen, H., Yang, T. Y., & Huang, H. C. (2017). Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes. Evidence-Based Complementary and Alternative Medicine, 2017,8325049. DOI: https://doi.org/10.1155/2017/8325049.
Dat, T. T. H., Oanh, P. T. T., Cuong, L. C. V., Anh, L. T., Minh, L. T. H., Ha, H., Lam, L. T., Cuong, P. V., & Anh, H. L. T. (2021). Pharmacological Properties, Volatile Organic Compounds, and Genome Sequences of Bacterial Endophytes from the Mangrove Plant Rhizophora apiculata Blume. Antibiotics, 10(12), 1491. DOI: https://doi.org/10.3390/antibiotics10121491.
Dat, T. T. H., Phuong, H. T., Tran, P. T. T., Souvannalath, B., Trung, H. L., Ho, D. V., & Viet, C. L. C. (2022). Secondary Metabolites From the Grasshopper-Derived Entomopathogenic Fungus Aspergillus Tamarii NL3 and Their Biological Activities. Natural Product Communications, 17(12), 1934578X221141548. DOI: https://doi.org/10.1177/1934578X221141548.
Guo, M.-F., Zhang, H.-H., Zhong, P., Xu, J.-D., Zhou, S.-S., Long, F., Kong, M., Mao, Q., & Li, S.-L. (2022). Integrating Multi-Type Component Determination and Anti-Oxidant/-Inflammatory Assay to Evaluate the Impact of Pre-Molting Washing on the Quality and Bioactivity of Cicadae Periostracum. Molecules, 27(22), 7683. DOI: https://doi.org/10.3390/molecules27227683.
Huang, L., Zhu, X., Zhou, S., Cheng, Z., Shi, K., Zhang, C., & Shao, H. (2021). Phthalic acid esters: Natural sources and biological activities. Toxins, 13(7), 495. DOI: https://doi.org/10.3390/toxins13070495.
Jin, X., Zhou, J., Richey, G., Wang, M., Choi Hong, S. M., & Hong, S. H. (2021). Undecanoic acid, lauric acid, and N-tridecanoic acid inhibit Escherichia coli persistence and biofilm formation. Journal of Microbiology and Biotechnology, 31(1), 130-136. DOI: https://doi.org/10.4014/JMB.2008.08027.
Liu, H., Yan, Y. M., Liao, L., Wang, S. X., Zhang, Y., & Cheng, Y. X. (2019). Cicadamides A and B, N-acetyldopamine dimers from the insect periostracum cicadae. Natural Product Communications, 14(6), 1934578X19850019. DOI: 10.1177/1934578X19850019
Nguyen, T. K., Thuy Thi Tran, L., Ho Viet, D., Thai, P. H., Ha, T. P., Ty, P. V., Duc, L. P., Ton That Huu, D., & Cuong, L. C. V. (2023). Xanthine oxidase, α-glucosidase and α-amylase inhibitory activities of the essential oil from Piper lolot: In vitro and in silico studies. Heliyon, 9(8), e19148. DOI: https://doi.org/10.1016/j.heliyon.2023.e19148.
Poerio, A., Petit, C., Jehl, J. P., Arab-Tehrany, E., Mano, J. F., & Cleymand, F. (2020). Extraction and physicochemical characterization of chitin from Cicada orni sloughs of the south-eastern French mediterranean basin. Molecules, 25(11), 2543. DOI: 10.3390/molecules25112543.
Price, B. W., Allan, E. L., Marathe, K., Sarkar, V., Simon, C., & Kunte, K. (2016). The cicadas (Hemiptera: Cicadidae) of India, Bangladesh, Bhutan, Myanmar, Nepal and Sri Lanka: an annotated provisional catalogue, regional checklist and bibliography. Biodiversity data journal, (4), e8051. DOI: https://doi.org/10.3897/BDJ.4.e8051.
Roy, R. N. (2020). Bioactive natural derivatives of phthalate ester. Critical Reviews in Biotechnology, 40(7), 913–929. DOI: https://doi.org/10.1080/07388551.2020.1789838.
Siddiqui, S. A., Li, C., Aidoo, O. F., Fernando, I., Haddad, M. A., Pereira, J. A. M., Blinov, A., Golik, A., & Câmara, J. S. (2023). Unravelling the potential of insects for medicinal purposes – A comprehensive review. Heliyon, 9(5). DOI: https://doi.org/10.1016/j.heliyon.2023.e15938.
Wu, S. J., Pan, S. K., Wang, H. Bin, & Wu, J. H. (2013). Preparation of chitooligosaccharides from cicada slough and their antibacterial activity. International Journal of Biological Macromolecules, 62, 348-351. DOI: https://doi.org/10.1016/j.ijbiomac.2013.09.042.
Xie, X., Guo, H., Liu, J., Wang, J., Li, H., & Deng, Z. (2023). Edible and Medicinal Progress of Cryptotympana atrata (Fabricius) in China. Nutrients, 15(19), 4266. DOI: https://doi.org/10.3390/nu15194266.
Xu, M. Z., Lee, W. S., Han, J. M., Oh, H. W., Park, D. S., Tian, G. R., Jeong, T. S., & Park, H. Y. (2006). Antioxidant and anti-inflammatory activities of N-acetyldopamine dimers from Periostracum cicadae. Bioorganic and Medicinal Chemistry, 14(23), 7826-7834. DOI: https://doi.org/10.1016/j.bmc.2006.07.063.
Yali, W., Aiying, L., & Maofa, Y. (2015). Fatty Acid Composition of Cicada Slough. Chemistry of Natural Compounds, 51, 1147–1148. DOI: https://doi.org/ 10.1007/s10600-015-1512-4.
Yang, L., Li, G. Y., Wang, H. Y., Zhang, K., Zhu, Y., Zhao, W., Wang, H., & Wang, J. H. (2016). Five new N-acetyldopamine dimers from Periostracum Cicadae. Phytochemistry Letters, 16, 97-102. DOI: https://doi.org/10.1016/j.phytol.2016.02.010.