Nghiên cứu thử nghiệm nuôi cấy mô rong câu (Gracilaria tenuistipitata) trong phòng thí nghiệm

Abstract

Seed stock of Gracilaria tenuistipitata plays a crucial role in enhancing the quality and yield of agar. In Vietnam, the quality of Gracilaria seed has been declining due to genetic degeneration, which in turn reduce growth performance, pathogen resistance and quality of harvested seaweed. The application of biotechnology such as tissue culture techniques is a potential solution for improving the seed quality of G. tenuistipitata. The results of this study indicated that Gracilaria tenuistipitata well adapted to tissue culture conditions at (salinity of 15 ‰, temperature of 27± 2℃, and a 12 h light : 12 h dark photoperiod). The sizes of seaweed (5 mm, 10 mm, and 15 mm) have significant effects on shoot regeneration and elongation of the thalli. After 20 days of cultivation, the numbers of newly formed shoots in the three size classes were 4.82 ± 0.91, 17.89 ± 1.12, and 31.07 ± 2.51 shoots, respectively. Growth regulator treatments (NAA and BAP at 0.1, 0.3, and 0.5 mg/L) supported growth, but the number of newly regenerated shoots was consistently lower than in media without supplementation. In both control samples and those cultured in MS medium, shoot number and thallus length increased steadily across successive observations. These findings highlight the potential of tissue culture propagation to restore Gracilaria seed quality and to provide a sustainable source of large-scale seedlings for commercial seaweed farming.