##plugins.themes.huaf_theme.article.main##
Abstract
This study investigated the polymorphism of the Leptin (LEP) and Thyroglobulin (TG5) genes in 31 Droughtmaster × Hybrid Brahman cattle raised in Hue city. With the same crossbred cattle group, genetic characteristics may differ between different geographical regions. Therefore, this study is essential to provide baseline data for developing effective molecular marker-based selection strategies for local beef cattle herds. The PCR-RFLP method was employed to identify the single nucleotide polymorphisms (SNP) of LEP/Sau3AI (intron 2) and TG5/PsuI (5’UTR). The results showed that, three genotypes of LEPAA, LEPAB and LEPBB were detected, with corresponding allele frequencies of 0.68 for LEPA and 0.32 for LEPB. The population is in Hardy-Weinberg equilibrium at SNP LEP/Sau3AI. In contrast, for the TG5 gene, only 2 genotypes, TG5CC and TG5CT, were detected. The allele frequencies for TG5C and TG5T were = 0.53 and= 0.47, respectively. This population was not in Hardy-Weinberg equilibrium with a very low effective allele number (Ne) (1.22). The polymorphism levels of both genes were classified as intermediate, with the PIC index ranging from 0.34 to 0.37. Single nucleotide polymorphisms of LEP/Sau3AI and TG5/PsuI in Droughtmaster × Hybrid Brahman cattle are considered as the initial scientific basis to evaluate the potential application of molecular markers in local beef cattle breeding.
##plugins.themes.huaf_theme.article.details##
How to Cite
Ho Le Quynh Chau, Hoang Huu Tinh, Duong Thi Huong, Vu Tuan Minh, Le Van Dat, Truong Thi Huyen An, Nguyen Dang Trung, Kongvilay Sayasane, & Phan Thi Duy Thuan. (2026). Polymorphism of LEPTIN and THYROGLOBULIN genes in Droughtmaster × Hybrid Brahman cattle raised in Hue city. E-Journal of Agricultural Science and Technology, 10(1), 5332–5340. https://doi.org/10.46826/huaf-jasat.v10n1y2026.1333
Section
ANIMAL HUSBANDRY - VETERINARY MEDICINE - AQUACULTURE- ANIMAL SCIENCES
Copyright @ 2017-2026 by E-Journal of Agricultural Science and Technology
References
Hồ Lê Quỳnh Châu, Dương Thị Hương, Thân Thị Thanh Trà và Đinh Văn Dũng. (2023). Đa hình gen Leptin và Thyroglobulin liên quan đến tính trạng năng suất và phẩm chất thịt ở tổ hợp bò lai Senepol và lai Brahman nuôi tại tỉnh Thừa Thiên Huế. Tạp chí Khoa học Đại học Huế: Nông nghiệp và Phát triển Nông thôn, 132(3B), 191-203. https://doi.org/10.26459/hueunijard.v132i3B.7157
Sở Khoa học và Công nghệ thành phố Huế. (28/05/2022). Ứng dụng tiến bộ Khoa học và Công nghệ phát triển đàn bò lai chuyên thịt trên nền bò cái lai Zebu. Trang thông tin điện tử Sở Khoa học và Công nghệ thành phố Huế. Khai thác từ https://skhcn.hue.gov.vn/hoat-dong-kh-cn-dia-phuong/ung-dung-tien-bo-kh-cn-phat-trien-dan-bo-lai-chuyen-thit-tren-nen-bo-cai-lai-zebu.html.
Almeida, S. E. M., Almeida, E. A., Moraes, J. F. C, & Weimer, T.A. (2003). Molecular markers in LEP gene and reproductive performance of beef cattle. Journal of Animal Breeding and Genetics, 120(2), 106-113. https://doi.org/ 10.1046/j.1439- 0388.2003.00377.
Anwar, S., Putra A. C., Wulandari, A. S., Agung, P. P., Putra, W. P. B., & Said, S. (2017). Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia. Journal of the Indonesian Tropical Animal Agriculture, 42(3), 175-184. https://doi.org/ 10.14710/jitaa.42.3.175-184.
Casas, E., White, S. N., Riley, D. G., Smith, T. P., Brenneman, R. A., Olson, T. A., Johnson, D. D., Coleman, S. W., Bennett, G. L., & Chase, C. C. Jr. (2005). Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. Journal of Animal Science, 83(1), 13–19. https://doi.org/ 10.2527/2005.83113x.
Casas, E., White, S. N., Shackelford, S. D., Wheeler, T. L., Koohmaraie, M., & Bennett, G. L. (2007). Assessing the association of single nucleotide polymorphisms at the thyroglobulin gene with carcass traits in beef cattle. Journal of Animal Science, 85(11), 2807–2814. https://doi.org/10.2527/jas.2007-0179.
Chau, H. L. Q., Tra, T. T. T., Huong, D. T., Hang L. T. T, & Dung, D. V. (2024). The polymorphism of Leptin and Thyroglobulin genes in Lai Sind and Lai Brahman cattle. Journal of Animal and Feed Reasearch, 14(3), 165-170. https://doi.org/10.51227/ojafr.2024.20
Dung, D. V., Huong, D. T., Tra, T. T. T., Hang, L. T. T, Phung, L. D., Van, N. H., & Chau, H. L. Q. (2024). Genetic characterization of LEP and TG5 gene polymorphisms in crossbred beef cattle populations. Journal of advanced veterinary and animal research, 11(4), 989–995. https://doi.org/10.5455/javar.2024.k849.
De Carvalho, T. D., Siqueira, F., de Almeida Torres Júnior, R. A., de Medeiros, S. R., Feijó, G. L. D., de Souza Junior, M. D., Blecha, I. M. Z, & Soares, C. O. (2012). Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle. Revista Brasileira de Zootecnia, 41(10), 2162-2168. DOI: 10.1590/S1516-35982012001000004.
Dolmatova, I., Sedykh, T., Valitov, F., Gizatullin, R., Khaziev, D., & Kharlamov, A. (2020), Effect of the bovine TG5 gene polymorphism on milk- and meat-producing ability. Veterinary World, 13(10), 2046–2052. https://doi.org/10.14202/vetworld.2020.2046-2052.
Don, N. V., Oanh, N. C., & Aduli, M. E. O. (2021). Main regulatory factors of marbling level in beef cattle. Veterinary and Animal Science, 14, 100219. DOI: 10.1016/j.vas.2021.100219.
Fortes, M. R., Curi, R. A., Chardulo, L. A., Silveira, A. C., Assumpção, M. E., Visintin, J. A., & Oliveira, H. N. (2009). Bovine gene polymorphisms related to fat deposition and meat tenderness. Genetics and Molecular Biology, 32(1), 75–82. https://doi.org/10.1590/S1415-47572009000100011.
Javanmard, A., Mohammadabadi, M. R., Zarrigabayi, G. E., Gharahedaghi, A. A., Nassiry, M. R, Javadmansh, A., & Asadzadeh, N. (2008). Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russian Journal of Genetics, 44(4), 495–497.
Hussain, D. A., Abboud, Z. H., & Abdulameer, T. A. (2017). Genetic structure analysis of leptin gene/ Sau3AI and its relationship with body weigh in Iraqi and Holstein Frisian cows population (Comparative study). IOSR Journal of Pharmacy and Biological Sciences, 12(3), 10–13. https://doi.org/10.9790/3008-1203041013.
Kök, S. &Vapur, G. (2021). Effects of leptin and thyroglobulin gene polymorphisms on beef quality in Holsteinbreed bulls in Turkey. Turkish Journal of Veterinary & Animal Sciences, 45(2), 238-247. https://doi.org/10.3906/vet-2009-12.
Liefers, S. C., te Pas, M. F. W., Veerkamp, R. F. & van der Lende, T. (2002). Associations between Leptin gene polymorphisms and production, live weight, energy balance, feed intake and fertility in Holstein heifers. Journal of Dairy Science, 85, 1633–1638. https://doi.org/10.3168/jds.S0022-0302(02)74235-5.
Nei, M. & Kumar, S. (2000). Molecular evolution and phylogenetics. Oxford University Press, New York. Retrieved from https://global.oup.com/academic/product/molecular-evolution-and-phylogenetics-9780195135855?cc=vn&lang=en&.
Mears, G. J., Mir, P. S., Bailey, D. R. C., & Jones, S. D. M. (2001). Effect of Wagyu genetics on marbling, backfat and circulating hormones in cattle. Canadian Journal of Animal Science, 81, 65-73. https://doi.org/10.4141/A99-128.
Milan, M., Nevres, P., Miodrag, L., Aida, H. Vesna, D., Ljubodrag, S., & Zoran, S. (2019). Insight in leptin gene polymorphism and impact on milk traits in autochtonous Busha cattle. Acta Veterinaria-Beograd, 69(2), 153-163. https://doi.org/10.2478/acve-2019-0012.
Pannier, L., Mullen, A. M., Hamill, R. M., Stapleton, P. C., & Sweeney, T. (2010). Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat Science, 85(3), 515–518. https://doi.org/10.1016/j.meatsci.2010.02.025.
Putra, W. P. B., Anwar, S., Said, S., Indratno, S. A. A., & Wulandari, P. (2019). Genetic characterization of Thyroglobulin and Leptin genes in Pasundan cattle at West Java. Buletin Peternakan, 43(1), 1–7. https://doi.org/10.21059/buletinpeternak.v43i1.38227.
Rivera-Prieto, A. R., Garza-Hernandez, D., Torres-Grimaldo, A. A., Cardenas-Ramos, S. G., Reyes-Cortes, L. M., Karr-de-Leon, S. F., & Barrera- Saldana, H. A. (2015). Tenderness and marbling-related polymorphisms in beefmaster cattle. Asian Journal of Animal and Veterinary Advances, 10(7), 345-351. https://doi.org/10.3923/ajava.2015.345.351.
Roychoudhury, A. K. & Nei, M. (1988). Human polymorphic genes world distributions. New York: Oxford University Press. https://doi.org/10.1002/ajpa.1330790412.
Schumacher, M., DelCurto-Wyffels, H., Thomson, J., & Boles, J. (2022). Fat deposition and fat effects on meat quality - A review. Animals (Basel), 12(12),1550. https://doi.org/10.3390/ani12121550.
Sedykh, T. A., Kalashnikova, L. A., Gusev, I. V., Pavlova, I. Y., Gizatullin, R. S., & Dolmatova, I. Y. (2016). Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves. Iraqi Journal of Veterinary Sciences, 30(2), 41-48. https://doi.org/ 10.33899/ijvs.2016.121382
Sharifzadeh, A. & Doosti, A. (2010). Genetic polymorphism at the leptin gene in Iranian Holstein cattle by PCR-RFLP. African Journal of Microbiology Research, 4(12), 1343-1345.
Sycheva, I., Latynina, E., Mamedov, A., Tsibizova, O., Kozak, Y., Svistounov, D., Bystrenina, I., & Orishev A. (2023). Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls. Veterinary World, 16(8), 1647–1654.
Thaller, G., Kuhn, C., Winter, A., Ewald, G., Bellmann, O., Wegner, J., Zuhlke, H., & Fries, R. (2003). DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Animal Genetics, 34, 354–357. https://doi.org/10.1046/j.1365-2052.2003.01011.x.
Yang, D., Chen, H., Wang, X., Tian, Z., Tang, L., Zhang, Z., & Zhang, L. (2007). Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. Journal of Genetics and Genomics, 34, 400-405. https://doi.org/10.1016/S1673-8527(07)60043-5.
Sở Khoa học và Công nghệ thành phố Huế. (28/05/2022). Ứng dụng tiến bộ Khoa học và Công nghệ phát triển đàn bò lai chuyên thịt trên nền bò cái lai Zebu. Trang thông tin điện tử Sở Khoa học và Công nghệ thành phố Huế. Khai thác từ https://skhcn.hue.gov.vn/hoat-dong-kh-cn-dia-phuong/ung-dung-tien-bo-kh-cn-phat-trien-dan-bo-lai-chuyen-thit-tren-nen-bo-cai-lai-zebu.html.
Almeida, S. E. M., Almeida, E. A., Moraes, J. F. C, & Weimer, T.A. (2003). Molecular markers in LEP gene and reproductive performance of beef cattle. Journal of Animal Breeding and Genetics, 120(2), 106-113. https://doi.org/ 10.1046/j.1439- 0388.2003.00377.
Anwar, S., Putra A. C., Wulandari, A. S., Agung, P. P., Putra, W. P. B., & Said, S. (2017). Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia. Journal of the Indonesian Tropical Animal Agriculture, 42(3), 175-184. https://doi.org/ 10.14710/jitaa.42.3.175-184.
Casas, E., White, S. N., Riley, D. G., Smith, T. P., Brenneman, R. A., Olson, T. A., Johnson, D. D., Coleman, S. W., Bennett, G. L., & Chase, C. C. Jr. (2005). Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. Journal of Animal Science, 83(1), 13–19. https://doi.org/ 10.2527/2005.83113x.
Casas, E., White, S. N., Shackelford, S. D., Wheeler, T. L., Koohmaraie, M., & Bennett, G. L. (2007). Assessing the association of single nucleotide polymorphisms at the thyroglobulin gene with carcass traits in beef cattle. Journal of Animal Science, 85(11), 2807–2814. https://doi.org/10.2527/jas.2007-0179.
Chau, H. L. Q., Tra, T. T. T., Huong, D. T., Hang L. T. T, & Dung, D. V. (2024). The polymorphism of Leptin and Thyroglobulin genes in Lai Sind and Lai Brahman cattle. Journal of Animal and Feed Reasearch, 14(3), 165-170. https://doi.org/10.51227/ojafr.2024.20
Dung, D. V., Huong, D. T., Tra, T. T. T., Hang, L. T. T, Phung, L. D., Van, N. H., & Chau, H. L. Q. (2024). Genetic characterization of LEP and TG5 gene polymorphisms in crossbred beef cattle populations. Journal of advanced veterinary and animal research, 11(4), 989–995. https://doi.org/10.5455/javar.2024.k849.
De Carvalho, T. D., Siqueira, F., de Almeida Torres Júnior, R. A., de Medeiros, S. R., Feijó, G. L. D., de Souza Junior, M. D., Blecha, I. M. Z, & Soares, C. O. (2012). Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle. Revista Brasileira de Zootecnia, 41(10), 2162-2168. DOI: 10.1590/S1516-35982012001000004.
Dolmatova, I., Sedykh, T., Valitov, F., Gizatullin, R., Khaziev, D., & Kharlamov, A. (2020), Effect of the bovine TG5 gene polymorphism on milk- and meat-producing ability. Veterinary World, 13(10), 2046–2052. https://doi.org/10.14202/vetworld.2020.2046-2052.
Don, N. V., Oanh, N. C., & Aduli, M. E. O. (2021). Main regulatory factors of marbling level in beef cattle. Veterinary and Animal Science, 14, 100219. DOI: 10.1016/j.vas.2021.100219.
Fortes, M. R., Curi, R. A., Chardulo, L. A., Silveira, A. C., Assumpção, M. E., Visintin, J. A., & Oliveira, H. N. (2009). Bovine gene polymorphisms related to fat deposition and meat tenderness. Genetics and Molecular Biology, 32(1), 75–82. https://doi.org/10.1590/S1415-47572009000100011.
Javanmard, A., Mohammadabadi, M. R., Zarrigabayi, G. E., Gharahedaghi, A. A., Nassiry, M. R, Javadmansh, A., & Asadzadeh, N. (2008). Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bos taurus). Russian Journal of Genetics, 44(4), 495–497.
Hussain, D. A., Abboud, Z. H., & Abdulameer, T. A. (2017). Genetic structure analysis of leptin gene/ Sau3AI and its relationship with body weigh in Iraqi and Holstein Frisian cows population (Comparative study). IOSR Journal of Pharmacy and Biological Sciences, 12(3), 10–13. https://doi.org/10.9790/3008-1203041013.
Kök, S. &Vapur, G. (2021). Effects of leptin and thyroglobulin gene polymorphisms on beef quality in Holsteinbreed bulls in Turkey. Turkish Journal of Veterinary & Animal Sciences, 45(2), 238-247. https://doi.org/10.3906/vet-2009-12.
Liefers, S. C., te Pas, M. F. W., Veerkamp, R. F. & van der Lende, T. (2002). Associations between Leptin gene polymorphisms and production, live weight, energy balance, feed intake and fertility in Holstein heifers. Journal of Dairy Science, 85, 1633–1638. https://doi.org/10.3168/jds.S0022-0302(02)74235-5.
Nei, M. & Kumar, S. (2000). Molecular evolution and phylogenetics. Oxford University Press, New York. Retrieved from https://global.oup.com/academic/product/molecular-evolution-and-phylogenetics-9780195135855?cc=vn&lang=en&.
Mears, G. J., Mir, P. S., Bailey, D. R. C., & Jones, S. D. M. (2001). Effect of Wagyu genetics on marbling, backfat and circulating hormones in cattle. Canadian Journal of Animal Science, 81, 65-73. https://doi.org/10.4141/A99-128.
Milan, M., Nevres, P., Miodrag, L., Aida, H. Vesna, D., Ljubodrag, S., & Zoran, S. (2019). Insight in leptin gene polymorphism and impact on milk traits in autochtonous Busha cattle. Acta Veterinaria-Beograd, 69(2), 153-163. https://doi.org/10.2478/acve-2019-0012.
Pannier, L., Mullen, A. M., Hamill, R. M., Stapleton, P. C., & Sweeney, T. (2010). Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat Science, 85(3), 515–518. https://doi.org/10.1016/j.meatsci.2010.02.025.
Putra, W. P. B., Anwar, S., Said, S., Indratno, S. A. A., & Wulandari, P. (2019). Genetic characterization of Thyroglobulin and Leptin genes in Pasundan cattle at West Java. Buletin Peternakan, 43(1), 1–7. https://doi.org/10.21059/buletinpeternak.v43i1.38227.
Rivera-Prieto, A. R., Garza-Hernandez, D., Torres-Grimaldo, A. A., Cardenas-Ramos, S. G., Reyes-Cortes, L. M., Karr-de-Leon, S. F., & Barrera- Saldana, H. A. (2015). Tenderness and marbling-related polymorphisms in beefmaster cattle. Asian Journal of Animal and Veterinary Advances, 10(7), 345-351. https://doi.org/10.3923/ajava.2015.345.351.
Roychoudhury, A. K. & Nei, M. (1988). Human polymorphic genes world distributions. New York: Oxford University Press. https://doi.org/10.1002/ajpa.1330790412.
Schumacher, M., DelCurto-Wyffels, H., Thomson, J., & Boles, J. (2022). Fat deposition and fat effects on meat quality - A review. Animals (Basel), 12(12),1550. https://doi.org/10.3390/ani12121550.
Sedykh, T. A., Kalashnikova, L. A., Gusev, I. V., Pavlova, I. Y., Gizatullin, R. S., & Dolmatova, I. Y. (2016). Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves. Iraqi Journal of Veterinary Sciences, 30(2), 41-48. https://doi.org/ 10.33899/ijvs.2016.121382
Sharifzadeh, A. & Doosti, A. (2010). Genetic polymorphism at the leptin gene in Iranian Holstein cattle by PCR-RFLP. African Journal of Microbiology Research, 4(12), 1343-1345.
Sycheva, I., Latynina, E., Mamedov, A., Tsibizova, O., Kozak, Y., Svistounov, D., Bystrenina, I., & Orishev A. (2023). Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls. Veterinary World, 16(8), 1647–1654.
Thaller, G., Kuhn, C., Winter, A., Ewald, G., Bellmann, O., Wegner, J., Zuhlke, H., & Fries, R. (2003). DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Animal Genetics, 34, 354–357. https://doi.org/10.1046/j.1365-2052.2003.01011.x.
Yang, D., Chen, H., Wang, X., Tian, Z., Tang, L., Zhang, Z., & Zhang, L. (2007). Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. Journal of Genetics and Genomics, 34, 400-405. https://doi.org/10.1016/S1673-8527(07)60043-5.