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Abstract
Bacteriocin has been used widely in industry as a bio-preservative and an antimicrobial agent. The objective of the present study was to investigate the activity of bacteriocin produced by lactic acid bacteria (LAB) isolated from the fermented pinspotted spinefoot fish sauce of Hue as an antimicrobial of Vibrio infection in fish. The antimicrobial activity of bacteriocin against Vibrio alginiliticus YH18 and V. fluvialis YH6 were carried out by agar well difusion method on MRS medium. Results of this study showed that bacteriocins produced by the bacterial isolate R3 indicated the highest bacteriocin activity against V. fluvialis YH6 and V. alginiliticus YH18 at the diameter inhibition zone of 27,0 ± 2,2 mm and 20,0 ± 1,3 mm, respectively. While isolate R16 indicated the lowest diameter inhibition zone of 8,0 ± 1,2 mm with V. alginolyticus YH18. Results of this study showed that bacterial isolates R3, R4 and R16 have the potential to produce bacteriocins that inhibit the pathogen bacteria V. alginolyticus YH18 and V. fluvialis YH6. The bacterial isolates R3, R4 và R16 were indicated as Lactobacillus fermentum by MALDI-TOF MS identification method.
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How to Cite
Nguyen Thi Xuan Hong, Nguyen Thi Hue Linh, Nguyen Duc Quynh Anh, Do Thi Bich Thuy, Pham Thi Hai Yen, Nguyen Thi Dao, & Nguyen Nam Quang. (2025). Investigation of bacteriocin activity of lactic acid bacterial strains isolated from the fermented pinspotted spinefoot fish sauce of Hue. E-Journal of Agricultural Science and Technology, 9(1), 4663–4671. https://doi.org/10.46826/huaf-jasat.v9n1y2025.1192
Section
ANIMAL SCIENCE - VETERINARY MEDICINE - FISHERIES - ANIMALS
Copyright @ 2017-2025 by E-Journal of Agricultural Science and Technology
References
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Anjana, A., & Tiwari, S. K. (2022). Bacteriocin-producing probiotic lactic acid bacteria in controlling dysbiosis of the gut microbiota. Frontiers in Cellular and Infection Microbiology, 12, 851140.
Axelsson, L. (2004). Lactic acid bacteria: classification and physiology. Food Science and Technology-New York-Marcel Dekker, 139, 1-66.
Chen, X., Liu, H., Liu, S., & Mao, J. (2024). Impact of bacteriocins on multidrug‐resistant bacteria and their application in aquaculture disease prevention and control. Reviews in Aquaculture.
Cho, G. S., & Do, H. K. (2006). Isolation and identification of lactic acid bacteria isolated from a traditional jeotgal product in Korea. Ocean science journal, 41, 113-119.
Cotter, P. D., Ross, R. P., Hill, C. (2013). Bacteriocins—A Viable Alternative to Antibiotics? Nature Review Genetics, 11, 95–105.
Doan, N. T. L., K, Van Hoorde., Cnockaert, M., De Brandt, E., Aerts, M., Le, Thanh B., & Vandamme, P. (2012). Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam. Letters in Applied Microbiology, 55, 265-273.
Faikoh, E. N., Hong, Y. H., & Hu., S. Y. (2014). Liposome-encapsulated cinnamaldehyde enhances zebrafish (Danio rerio) immunity and survival when challenged with Vibrio vulnificus and Streptococcus agalactiae. Fish & Shellfish Immunology, 38, 15-24
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Hammami, R., Zouhir, A., Ben Hamida, J., & Fliss, I. (2007). Bactibase: A New Web-Accessible Database for Bacteriocin Characterization. BMC Microbiology, 7, 1-6.
Hernández-González, J. C., Martínez-Tapia, A., Lazcano-Hernández, G., García-Pérez, B. E., Castrejón-Jiménez, N.S. (2021). Bacteriocins from lactic acid bacteria. A powerful alternative as antimicrobials, probiotics, and immunomodulators in veterinary medicine. Animals, 11, 979.
Hertzberger, R., Arents, J., Dekker, H. L., Pridmore, R. D., Gysler, C., Kleerebezem, M., de Mattos, M. J. T.,(2014). H2O2 production in species of the Lactobacillus acidophilus group: a central role for a novel NADH-dependent flavin reductase. Applied and environmental microbiology, 80, 2229-2239.
Kassaa, I. A., Rafei, R., Moukhtar, M., Zaylaa, M., Gharsallaoui, A., Asehraou, A., El Omari, K., Shahin, A., Hamze, M., & Chihib, N. E. (2019). LABiocin database: A new database designed specifically for Lactic Acid Bacteria bacteriocins. International Journal of antimicrobial agents, 54(6), 71-779.
Lulijwa, R., Rupia, E. J., & Alfaro, A. C. (2020). Antibiotic use in aquaculture, policies and regulation, health and environmental risks: a review of the top 15 major producers. Reviews in Aquaculture, 12(2), 640-663.
Lv, X., Du, J., Jie, Y., Zhang, B., Fengling, B., & Zhao, H., Li, J. (2017). Purification and Antibacterial Mechanism of Fish-Borne Bacteriocin and Its Application in Shrimp (Penaeus vannamei) for Inhibiting Vibrio parahaemolyticus. World Journal of Microbiology and Biotechnology, 33, 156.
Pereira, W. A., Mendonça, C. M. N., Urquiza, A. V., Marteinsson, V. Þ., LeBlanc, J. G., Cotter, P. D., Villalobos, E. F., Romero, J., & Oliveira, R. P. (2022). Use of probiotic bacteria and bacteriocins as an alternative to antibiotics in aquaculture. Microorganisms, 10, 1705.
Ponce, A. G., Moreira, M. R., Del Valle, C. E., & Roura, S. I. (2008). Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables. LWT Food Science and Technology (Campinas.), 41, 432–441.
Senthamarai, M. D., Rajan, M. R., & Bharathi, P. V. (2023). Current risks of microbial infections in fish and their prevention methods: A review. Microbiology Pathogenesis, 185, 106400
Nguyễn Thị Xuân Hồng, Nguyễn Ngọc Phước, Nguyễn Thị Huế Linh, Đỗ Thị Bích Thuỷ, Nguyễn Thị Diễm Hương và Nguyễn Thị Thu Giang. (2022). Một số chủng vi khuẩn lactic tiềm năng phân lập từ mắm cá cơm được sử dụng làm probiotic trong nuôi trồng thuỷ sản. Tạp chí khoa học Đại học Huế, 131(3), 49-61.
Ahmad, V., Khan, M. S., Jamal, Q. M. S., Alzohairy, M. A., Al Karaawi, M. A., & Siddiqui, M. U. (2017). Antimicrobial Potential of Bacteriocins: In Therapy, Agriculture and Food Preservation. International Journal of Antimicrobial Agents, 49, 1–11.
Anjana, A., & Tiwari, S. K. (2022). Bacteriocin-producing probiotic lactic acid bacteria in controlling dysbiosis of the gut microbiota. Frontiers in Cellular and Infection Microbiology, 12, 851140.
Axelsson, L. (2004). Lactic acid bacteria: classification and physiology. Food Science and Technology-New York-Marcel Dekker, 139, 1-66.
Chen, X., Liu, H., Liu, S., & Mao, J. (2024). Impact of bacteriocins on multidrug‐resistant bacteria and their application in aquaculture disease prevention and control. Reviews in Aquaculture.
Cho, G. S., & Do, H. K. (2006). Isolation and identification of lactic acid bacteria isolated from a traditional jeotgal product in Korea. Ocean science journal, 41, 113-119.
Cotter, P. D., Ross, R. P., Hill, C. (2013). Bacteriocins—A Viable Alternative to Antibiotics? Nature Review Genetics, 11, 95–105.
Doan, N. T. L., K, Van Hoorde., Cnockaert, M., De Brandt, E., Aerts, M., Le, Thanh B., & Vandamme, P. (2012). Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam. Letters in Applied Microbiology, 55, 265-273.
Faikoh, E. N., Hong, Y. H., & Hu., S. Y. (2014). Liposome-encapsulated cinnamaldehyde enhances zebrafish (Danio rerio) immunity and survival when challenged with Vibrio vulnificus and Streptococcus agalactiae. Fish & Shellfish Immunology, 38, 15-24
Feliatra, F., Muchlisin, Z. A., Teruna, H. Y., Utamy, W. R., Nursyirwani, N., & Dahliaty, A. (2018). Potential of Bacteriocins Produced by Probiotic Bacteria Isolated from Tiger Shrimp and Prawns as Antibacterial to Vibrio, Pseudomonas, and Aeromonas Species on Fish. F1000Research, 7, 415.
Hammami, R., Zouhir, A., Ben Hamida, J., & Fliss, I. (2007). Bactibase: A New Web-Accessible Database for Bacteriocin Characterization. BMC Microbiology, 7, 1-6.
Hernández-González, J. C., Martínez-Tapia, A., Lazcano-Hernández, G., García-Pérez, B. E., Castrejón-Jiménez, N.S. (2021). Bacteriocins from lactic acid bacteria. A powerful alternative as antimicrobials, probiotics, and immunomodulators in veterinary medicine. Animals, 11, 979.
Hertzberger, R., Arents, J., Dekker, H. L., Pridmore, R. D., Gysler, C., Kleerebezem, M., de Mattos, M. J. T.,(2014). H2O2 production in species of the Lactobacillus acidophilus group: a central role for a novel NADH-dependent flavin reductase. Applied and environmental microbiology, 80, 2229-2239.
Kassaa, I. A., Rafei, R., Moukhtar, M., Zaylaa, M., Gharsallaoui, A., Asehraou, A., El Omari, K., Shahin, A., Hamze, M., & Chihib, N. E. (2019). LABiocin database: A new database designed specifically for Lactic Acid Bacteria bacteriocins. International Journal of antimicrobial agents, 54(6), 71-779.
Lulijwa, R., Rupia, E. J., & Alfaro, A. C. (2020). Antibiotic use in aquaculture, policies and regulation, health and environmental risks: a review of the top 15 major producers. Reviews in Aquaculture, 12(2), 640-663.
Lv, X., Du, J., Jie, Y., Zhang, B., Fengling, B., & Zhao, H., Li, J. (2017). Purification and Antibacterial Mechanism of Fish-Borne Bacteriocin and Its Application in Shrimp (Penaeus vannamei) for Inhibiting Vibrio parahaemolyticus. World Journal of Microbiology and Biotechnology, 33, 156.
Pereira, W. A., Mendonça, C. M. N., Urquiza, A. V., Marteinsson, V. Þ., LeBlanc, J. G., Cotter, P. D., Villalobos, E. F., Romero, J., & Oliveira, R. P. (2022). Use of probiotic bacteria and bacteriocins as an alternative to antibiotics in aquaculture. Microorganisms, 10, 1705.
Ponce, A. G., Moreira, M. R., Del Valle, C. E., & Roura, S. I. (2008). Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables. LWT Food Science and Technology (Campinas.), 41, 432–441.
Senthamarai, M. D., Rajan, M. R., & Bharathi, P. V. (2023). Current risks of microbial infections in fish and their prevention methods: A review. Microbiology Pathogenesis, 185, 106400