Ảnh hưởng của bổ sung chế phẩm Bacillus Subtilis-b23 đến vi khuẩn đường ruột và biểu mô ruột non gà nòi sọc đen giai đoạn 1 - 42 ngày tuổi

Abstract

The study was conducted at the Center for Practice and Training on Animal Husbandry and Veterinary Medicine, Faculty of Animal Sciences and Veterinary Medicine, the University of Agriculture and Forestry, Hue University, to evaluate the effects of supplementing Bacillus subtilis-B23 on the intestinal microbiota and small intestinal epithelium of broilers aged 1–42 days. The results indicated that the gastrointestinal structure of the chickens developed positively, with an increase in the villus size in the small intestine of the experimental group supplemented with B. subtilis-B23, particularly in the ileum (p < 0,05), as observed through HE staining. Using the colony culture method, the findings revealed a statistically significant reduction in the number of intestinal microorganisms in the experimental chickens compared to the control group. Specifically, the number of E. coli bacteria reduced from 5.90Log10 CFU/g to 5.56Log10 CFU/g of feces (p < 0,05), while the B. subtilis count in the experimental group was higher (10.25Log10 CFU/ml) compared to the control group (9.78Log10 CFU/ml). However, regarding the total number of aerobic bacteria, the difference between the experimental group and the control group was not statistically significant. The results of this study indicated that the supplementation of Bacillus subtilis-B23 contributes to an increase in the gastrointestinal epithelial size and a reduction in the number of intestinal bacteria.