##plugins.themes.huaf_theme.article.main##
Abstract
It has been widely found that stress-rearing conditions of intensive tilapia farming in cages could trigger the release of catecholamine stress hormones, including norepinephrine, epinephrine and dopamine in aquatic animals. These hormones could enhance the growth and virulence factors of pathogenic bacteria. Thus, this study aimed to evaluate the effects of the stress hormone dopamine on the virulence of S. agalactiae in tilapia with two experiments: (1) impact of dopamine supplemented in enzymatic assays and biofilm formation of S. agalactiae in in vitro and (2) challenge test in tialia towards pre-treated or non-treated S. agalactiae with dopamine. The results showed that dopamine (50, 100 and 200 µM) increased biofilm formation and enzymatic activities of lipase, phospholipase and haemolysin but decreased the casinolyitc activity in S. agalactiae compared to those assays in controls and treatments with dopamine 25 µM. Results from the challenge test confirmed that pre-treated S. agalactiae with dopamine 50 µM significantly enhanced the mortality of tilapia in comparison with untreated bacteria and control treatment.
##plugins.themes.huaf_theme.article.details##
How to Cite
Nguyen Duc Quynh Anh, Nguyen Nam Quang, Nguyen Thi Hue Linh, Nguyen Thi Xuan Hong, & Nguyen Ngoc Phuoc. (2025). Influence of dopamine on virulence factors of Streptococcus agalactiae in tilapia (Oreochromis sp.). E-Journal of Agricultural Science and Technology, 9(1), 4695–4705. https://doi.org/10.46826/huaf-jasat.v9n1y2025.1202
Section
ANIMAL SCIENCE - VETERINARY MEDICINE - FISHERIES - ANIMALS
Copyright @ 2017-2025 by E-Journal of Agricultural Science and Technology
References
Nguyễn Đức Quỳnh Anh, Nguyễn Thị Huế Linh, Nguyễn Nam Quang, Huỳnh Văn Vì và Nguyễn Ngọc Phước. (2024). Ảnh hưởng của epinephrine lên độc lực vi khuẩn Vibrio harveyi trên tôm thẻ chân trắng (Litopenaeus vannamei).Tạp chí Khoa học và Công nghệ Nông nghiệp- Đại học Nông Lâm, Đại học Huế, 8(1), 4015-4026.
Nguyễn Ngọc Phước, Nguyễn Thị Huế Linh và Trần Thị Nhật Anh. (2019). Phân lập và xác định một số đặc điểm sinh học các chủng Streptococus agalactiae gây bệnh trên cá rô phi đỏ (Oreochromis sp.) nuôi tại Thừa Thiên Huế. Tạp chí Khoa học và Công nghệ Nông nghiệp trường Đại học Nông Lâm, Đại học Huế, 3(3), 1591-1601.
Belay, T., Aviles, H., Vance, M., Fountain, K., & Sonnenfeld, G. (2003). Catecholamines and in vitro growth of pathogenic bacteria: enhancement of growth varies greatly among bacterial species. Life Sciences, 73(12), 1527-1535.
Cheng, W., Chieu, H. T., Ho, M. C., & Chen, J. C. (2006). Noradrenaline modulates the immunity of white shrimp Litopenaeus vannamei. Fish & shellfish immunology, 21(1), 11-19.
Cambronel, M., Tortuel, D., Biaggini, K., Maillot, O., Taupin, L., Réhel, K., Rincé, I., Muller, C., Hardouin, J., Feuilloley, M., Rodrigues, S., & Connil, N. (2019). Epinephrine affects motility, and increases adhesion, biofilm and virulence of Pseudomonas aeruginosa H103. Scientific reports, 9(1), 20203.
Gao, J., Xi, B., Chen, K., Song, R., Qin, T., Xie, J., & Pan, L. (2019). The stress hormone norepinephrine increases the growth and virulence of Aeromonas hydrophila. Microbiologyopen, 8(4), e00664.
Huang, D. B., & DuPont, H. L. (2005). Problem pathogens: extra-intestinal complications of Salmonella enterica serotype Typhi infection. The Lancet infectious diseases, 5(6), 341-348.
Istivan, T. S., & Coloe, P. J. (2006). Phospholipase A in Gram-negative bacteria and its role in pathogenesis. Microbiology, 152(Pt 5), 1263-1274.
Karavolos, M. H., Bulmer, D. M., Spencer, H., Rampioni, G., Schmalen, I., Baker, S., Pickard, D., Gray, J., Fookes, M., Winzer, K., Ivens, A., Dougan, G., Williams, P., & Khan, C. M. (2011). Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E. EMBO Rep, 12(3), 252-258.
Kinney, K. S., Austin, C. E., Morton, D. S., & Sonnenfeld, G. (1999). Catecholamine enhancement of Aeromonas hydrophila growth. Microbial Pathogenesis, 26(2), 85-91.
Komal, W., Fatima, S., Minahal, Q., & Liaqat, R. (2024). Investigating the optimum stocking density of tilapia (Oreochromis niloticus) for intensive production focused to in-pond raceway system. Science Progress, 107(2), 00368504241257128.
Konto-Ghiorghi, Y., Mairey, E., Mallet, A., Duménil, G., Caliot, E., Trieu-Cuot, P., & Dramsi, S. (2009). Dual role for pilus in adherence to epithelial cells and biofilm formation in Streptococcus agalactiae. PLoS pathogens, 5(5), e1000422.
Kuo, C.M., Hsu, C.R., Lin, C.Y (1995). Hyperglycaemic effects of dopamine in tiger shrimp, Penaeus monodon. Aquaculture, 135(1-3), 161-172.
Lesouhaitier, O., Veron, W., Chapalain, A., Madi, A., Blier, A. S., Dagorn, A., Connil, N., Chevalier, S., Orange, N., & Feuilloley, M. (2009). Gram-negative bacterial sensors for eukaryotic signal molecules. Sensors (Basel), 9(9), 6967-6990.
Li, W., Su, Y. L., Mai, Y. Z., Li, Y. W., Mo, Z. Q., & Li, A. X. (2014). Comparative proteome analysis of two Streptococcus agalactiae strains from cultured tilapia with different virulence. Veterinary Microbiology, 170(1-2), 135-143.
Lyte, M., Freestone, P. P., Neal, C. P., Olson, B. A., Haigh, R. D., Bayston, R., & Williams, P. H. (2003). Stimulation of Staphylococcus epidermidis growth and biofilm formation by catecholamine inotropes. The Lancet, 361(9352), 130-135.
Nakano, M., Takahashi, A., Sakai, Y., Kawano, M., Harada, N., Mawatari, K., & Nakaya, Y. (2007). Catecholamine‐induced stimulation of growth in Vibrio species. Letters in applied microbiology, 44(6), 649-653.
Pande, G. S. J., Suong, N. T., Bossier, P., & Defoirdt, T. (2014). The catecholamine stress hormones norepinephrine and dopamine increase the virulence of pathogenic Vibrio anguillarum and Vibrio campbellii. FEMS microbiology ecology, 90(3), 761-769.
Phuoc, N. N., Linh, N. T. H., Crestani, C., & Zadoks, R. N. (2021). Effect of strain and enviromental conditions on the virulence of Streptococcus agalactiae (Group B Streptococcus; GBS) in red tilapia (Oreochromis sp.). Aquaculture, 534, 736256.
Sharaff, F., & Freestone, P. (2011). Microbial endocrinology. Central European Journal of Biology, 6, 685-694.
Reiche, E. M. V., Morimoto, H. K., & Nunes, S. M. V. (2005). Stress and depression-induced immune dysfunction: implications for the development and progression of cancer. International Review of Psychiatry, 17(6), 515-527.
Romalde, J. L., & Toranzo, A. E. (1993). Pathological activities of Yersinia ruckeri, the Enteric Redmouth (ERM) bacterium. FEMS microbiology letters, 112(3), 291-299.
Ramona, Y., Darmayasa, I. B. G., Widiantari, N. P., Shanti, N. N. B. D., Hani, N. L., Julyantoro, P. G. S., Oktariani, A.F., & Shetty, K. (2024). Catecholamines (dopamine) increases the virulence of Aeromonas hydrophila ATCC AH-1N, the causative agent of motile Aeromonas septicemia (MAS). 한국미생물· 생명공학회지, 52(2), 179-188.
Romana-Eguia, M. R. R., Eguia, R. V., & Pakingking Jr, R. V. (2020). Tilapia culture: The basics. Aquaculture Department, Southeast Asian Fisheries Development Center.
Sharaff, F., & Freestone, P. (2011). Microbial Endocrinology. Central European Journal of Biology, 6(5), 685-694.
Torabi Delshad, S., Soltanian, S., Sharifiyazdi, H., & Bossier, P. (2019). Effect of catecholamine stress hormones (dopamine and norepinephrine) on growth, swimming motility, biofilm formation and virulence factors of Yersinia ruckeri in vitro and an in vivo evaluation in rainbow trout. Journal of Fish Diseases, 42(4), 477-487.
Yang, Q., Anh, N. D.Q., Bossier, P., & Defoirdt, T. (2014). Norepinephrine and dopamine increase motility, biofilm formation, and virulence of Vibrio harveyi. Frontiers in microbiology, 5, 584.
Yang, Q., Zou, P., Cao, Z., Wang, Q., Fu, S., Xie, G., & Huang, J. (2021). QseC inhibition as a novel antivirulence strategy for the prevention of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus. Frontiers in Cellular and Infection Microbiology, 10, 594652.
Nguyễn Ngọc Phước, Nguyễn Thị Huế Linh và Trần Thị Nhật Anh. (2019). Phân lập và xác định một số đặc điểm sinh học các chủng Streptococus agalactiae gây bệnh trên cá rô phi đỏ (Oreochromis sp.) nuôi tại Thừa Thiên Huế. Tạp chí Khoa học và Công nghệ Nông nghiệp trường Đại học Nông Lâm, Đại học Huế, 3(3), 1591-1601.
Belay, T., Aviles, H., Vance, M., Fountain, K., & Sonnenfeld, G. (2003). Catecholamines and in vitro growth of pathogenic bacteria: enhancement of growth varies greatly among bacterial species. Life Sciences, 73(12), 1527-1535.
Cheng, W., Chieu, H. T., Ho, M. C., & Chen, J. C. (2006). Noradrenaline modulates the immunity of white shrimp Litopenaeus vannamei. Fish & shellfish immunology, 21(1), 11-19.
Cambronel, M., Tortuel, D., Biaggini, K., Maillot, O., Taupin, L., Réhel, K., Rincé, I., Muller, C., Hardouin, J., Feuilloley, M., Rodrigues, S., & Connil, N. (2019). Epinephrine affects motility, and increases adhesion, biofilm and virulence of Pseudomonas aeruginosa H103. Scientific reports, 9(1), 20203.
Gao, J., Xi, B., Chen, K., Song, R., Qin, T., Xie, J., & Pan, L. (2019). The stress hormone norepinephrine increases the growth and virulence of Aeromonas hydrophila. Microbiologyopen, 8(4), e00664.
Huang, D. B., & DuPont, H. L. (2005). Problem pathogens: extra-intestinal complications of Salmonella enterica serotype Typhi infection. The Lancet infectious diseases, 5(6), 341-348.
Istivan, T. S., & Coloe, P. J. (2006). Phospholipase A in Gram-negative bacteria and its role in pathogenesis. Microbiology, 152(Pt 5), 1263-1274.
Karavolos, M. H., Bulmer, D. M., Spencer, H., Rampioni, G., Schmalen, I., Baker, S., Pickard, D., Gray, J., Fookes, M., Winzer, K., Ivens, A., Dougan, G., Williams, P., & Khan, C. M. (2011). Salmonella Typhi sense host neuroendocrine stress hormones and release the toxin haemolysin E. EMBO Rep, 12(3), 252-258.
Kinney, K. S., Austin, C. E., Morton, D. S., & Sonnenfeld, G. (1999). Catecholamine enhancement of Aeromonas hydrophila growth. Microbial Pathogenesis, 26(2), 85-91.
Komal, W., Fatima, S., Minahal, Q., & Liaqat, R. (2024). Investigating the optimum stocking density of tilapia (Oreochromis niloticus) for intensive production focused to in-pond raceway system. Science Progress, 107(2), 00368504241257128.
Konto-Ghiorghi, Y., Mairey, E., Mallet, A., Duménil, G., Caliot, E., Trieu-Cuot, P., & Dramsi, S. (2009). Dual role for pilus in adherence to epithelial cells and biofilm formation in Streptococcus agalactiae. PLoS pathogens, 5(5), e1000422.
Kuo, C.M., Hsu, C.R., Lin, C.Y (1995). Hyperglycaemic effects of dopamine in tiger shrimp, Penaeus monodon. Aquaculture, 135(1-3), 161-172.
Lesouhaitier, O., Veron, W., Chapalain, A., Madi, A., Blier, A. S., Dagorn, A., Connil, N., Chevalier, S., Orange, N., & Feuilloley, M. (2009). Gram-negative bacterial sensors for eukaryotic signal molecules. Sensors (Basel), 9(9), 6967-6990.
Li, W., Su, Y. L., Mai, Y. Z., Li, Y. W., Mo, Z. Q., & Li, A. X. (2014). Comparative proteome analysis of two Streptococcus agalactiae strains from cultured tilapia with different virulence. Veterinary Microbiology, 170(1-2), 135-143.
Lyte, M., Freestone, P. P., Neal, C. P., Olson, B. A., Haigh, R. D., Bayston, R., & Williams, P. H. (2003). Stimulation of Staphylococcus epidermidis growth and biofilm formation by catecholamine inotropes. The Lancet, 361(9352), 130-135.
Nakano, M., Takahashi, A., Sakai, Y., Kawano, M., Harada, N., Mawatari, K., & Nakaya, Y. (2007). Catecholamine‐induced stimulation of growth in Vibrio species. Letters in applied microbiology, 44(6), 649-653.
Pande, G. S. J., Suong, N. T., Bossier, P., & Defoirdt, T. (2014). The catecholamine stress hormones norepinephrine and dopamine increase the virulence of pathogenic Vibrio anguillarum and Vibrio campbellii. FEMS microbiology ecology, 90(3), 761-769.
Phuoc, N. N., Linh, N. T. H., Crestani, C., & Zadoks, R. N. (2021). Effect of strain and enviromental conditions on the virulence of Streptococcus agalactiae (Group B Streptococcus; GBS) in red tilapia (Oreochromis sp.). Aquaculture, 534, 736256.
Sharaff, F., & Freestone, P. (2011). Microbial endocrinology. Central European Journal of Biology, 6, 685-694.
Reiche, E. M. V., Morimoto, H. K., & Nunes, S. M. V. (2005). Stress and depression-induced immune dysfunction: implications for the development and progression of cancer. International Review of Psychiatry, 17(6), 515-527.
Romalde, J. L., & Toranzo, A. E. (1993). Pathological activities of Yersinia ruckeri, the Enteric Redmouth (ERM) bacterium. FEMS microbiology letters, 112(3), 291-299.
Ramona, Y., Darmayasa, I. B. G., Widiantari, N. P., Shanti, N. N. B. D., Hani, N. L., Julyantoro, P. G. S., Oktariani, A.F., & Shetty, K. (2024). Catecholamines (dopamine) increases the virulence of Aeromonas hydrophila ATCC AH-1N, the causative agent of motile Aeromonas septicemia (MAS). 한국미생물· 생명공학회지, 52(2), 179-188.
Romana-Eguia, M. R. R., Eguia, R. V., & Pakingking Jr, R. V. (2020). Tilapia culture: The basics. Aquaculture Department, Southeast Asian Fisheries Development Center.
Sharaff, F., & Freestone, P. (2011). Microbial Endocrinology. Central European Journal of Biology, 6(5), 685-694.
Torabi Delshad, S., Soltanian, S., Sharifiyazdi, H., & Bossier, P. (2019). Effect of catecholamine stress hormones (dopamine and norepinephrine) on growth, swimming motility, biofilm formation and virulence factors of Yersinia ruckeri in vitro and an in vivo evaluation in rainbow trout. Journal of Fish Diseases, 42(4), 477-487.
Yang, Q., Anh, N. D.Q., Bossier, P., & Defoirdt, T. (2014). Norepinephrine and dopamine increase motility, biofilm formation, and virulence of Vibrio harveyi. Frontiers in microbiology, 5, 584.
Yang, Q., Zou, P., Cao, Z., Wang, Q., Fu, S., Xie, G., & Huang, J. (2021). QseC inhibition as a novel antivirulence strategy for the prevention of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus. Frontiers in Cellular and Infection Microbiology, 10, 594652.